Nanostructure, Effective Properties, and Deformation Pattern of the Cochlear Outer Hair Cell Cytoskeleton

[+] Author and Article Information
Alexander A. Spector, Mohammed Ameen, Aleksander S. Popel

Department of Biomedical Engineering and Center for Computational Medicine and Biology, Johns Hopkins University, 720 Rutland Ave., Baltimore, MD 21205

Panos G. Charalambides

Department of Mechanical Engineering, University of Maryland, Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250

J Biomech Eng 124(2), 180-187 (Mar 29, 2002) (8 pages) doi:10.1115/1.1448521 History: Received April 01, 2001; Revised November 01, 2001; Online March 29, 2002
Copyright © 2002 by ASME
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Geisler, C. D., 1998, From Sound to Synapse, Oxford University Press, New York.
Dallos, P., 1996, “Overview: Cochlear Neurobiology,” Dallos, P., Popper, A. N., and Fay R. R., eds., The Cochlea, Springer-Verlag, New York, pp. 1–43.
Santos-Sacchi,  J., 1993, “Harmonics of Outer Hair Cell Motility,” J. Neurosci., 12, pp. 1906–1916.
Brownell,  W. E., Bader,  C. R., Bertrand,  D., and de Ribaupierre,  Y., 1985, “Evoked Mechanical Responses of Isolated Cochlear Outer Hair Cell,” Science, 27, pp. 194–196.
Frank,  G., Hemmert,  W., and Gummer,  A. W., 1999, “Limiting Dynamics of High-Frequency Electromechanical Transduction of Outer Hair Cells,” Proc. Natl. Acad. Sci. U.S.A., 96, pp. 4420–4425.
Hallworth,  R., 1995, “Passive Compliance and Active Force Generation in the Guinea Pig Outer Hair Cell,” J. Neurosci., 74, pp. 2319–2328.
Evans,  B. N., and Dallos,  P., 1993, “Stereocilia Displacement Induced Somatic Motility of Outer Hair Cells,” Proc. Natl. Acad. Sci. U.S.A., 90, 8347–8351.
Xue, S., Mountain, D. C., and Hubbard, A. E., 1993, “Direct Measurements of Electrically-Evoked Basilar Membrane Motion,” Duinfuis, H., Horst, J. W., van Dijk, P., and van Netten, S. M. eds., Biophysics of Hair Cell Sensory Systems, World Scientific, Singapore, pp. 361–368.
Mammano,  F., and Ashmore,  J. F., 1993, “Reverse Transduction Measured in the Isolated Cochlea by Laser Michelson Interferometry,” Nature (London), 365, pp. 838–841.
Spector,  A. A., Ameen,  M., and Popel,  A. S., 2001, “Simulation of Motor-Driven Cochlear Outer Hair Cell Electromotility,” Biophys. J., 81, pp. 11–24.
Holley,  M. C., and Ashmore,  J. F., 1988, “A Cytoskeletal Spring in Cochlear Outer Hair Cells,” Nature (London), 335, pp. 635–637.
Forge,  A., 1991, “Structural Features of the Lateral Wall in Mammalian Cochlea Outer Hair Cells,” Cell Tissue Res., 265, pp. 473–484.
Holley,  M. C., Kalinec,  F., and Kachar,  B., 1992, “Structure of the Cortical Cytoskeleton in Mammalian Outer Hair Cells,” J. Cell. Sci., 102, pp. 569–580.
Tolomeo,  J. A., Steele,  C. R., and Holley,  M. C., 1996, “Mechanical Properties of the Lateral Cortex of Mammalian Auditory Outer Hair Cells,” Biophys. J., 71, pp. 421–429.
Tolomeo,  J. A., and Steele,  C. R., 1995, “Orthotropic Properties of the Composite Outer Hair Cell Wall,” J. Acoust. Soc. Am., 97, pp. 3006–3011.
Spector,  A. A., Brownell,  W. E., and Popel,  A. S., 1998, “Analysis of the Micropipet Experiment with the Anisotropic Outer Hair Cell Wall,” J. Acoust. Soc. Am., 103, pp. 1001–1006.
Spector,  A. A., Brownell,  W. E., and Popel,  A. S., 1998, “Estimation of Elastic Moduli and Bending Stiffness of Anisotropic Outer Hair Cell Wall,” J. Acoust. Soc. Am., 103, pp 1007–1011.
Adachi,  M., and Iwasa,  K. H., 1997, “Effect of Diamide on Force Generation and Axial Stiffness of the Cochlear Outer Hair Cell,” Biophys. J., 73, pp. 2809–2818.
Boal,  D. H., 1994, “Computer Simulation of a Model Network for the Erythrocyte Cytoskeleton,” Biophys. J., 67, pp. 521–529.
Hansen,  J. C., Skalak,  R., Chien,  S., and Hoger,  A., 1996, “An Elastic Network Model Based on the Structure of the Red Blood Cell Membrane Skeleton,” Biophys. J., 70, pp. 146–166.
Hansen,  J. C., Skalak,  R., Chien,  S., and Hoger,  A., 1997, “Influence of Network Topology on the Elasticity of the Red Blood Cell Membrane Skeleton,” Biophys. J., 72, pp. 2369–2381.
Hansen,  J. C., Skalak,  R., Chien,  S., and Hoger,  A., 1997, “Spectrin Properties and the Elasticity of the Red Blood Cell Membrane Skeleton,” Biorheology, 34, pp. 327–348.
Boey,  S. K., Beal,  D. H., and Discher,  D. E., 1998, “Simulation of the Erythrocyte Cytoskeleton at Large Deformation. I. Microscopic Models,” Biophys. J., 75, pp. 1573–1583.
Satcher,  R. L., and Dewey,  C. F., 1996, “Theoretical Estimates of Mechanical Properties of the Endothelial cell Cytoskeleton,” Biophys. J., 71, pp. 109–118.
Suciu,  A., Civelekoglu,  G., Tardy,  Y., and Meister,  J.-J., 1997, “Model for the Alignment of Actin Filaments in Endothelial Cells Subjected to Fluid Shear Stress,” Bull. Math. Biol., 59, pp. 1029–1046.
Civelekoglu,  G., and Edelstein-Keshet,  L., 1994, “Modelling the Dynamics of F-actin in the Cell,” Bull. Math. Biol., 56, pp. 587–616.
Kojima,  H., Ishijima,  A., and Yanagida,  T., 1994, “Direct Measurements of Stiffness of Single Actin Filaments with and without Thropomyosin by in Vitro Nanomanipulation,” Proc. Natl. Acad. Sci. U.S.A., 91, pp 12962–12966.
Gittes,  F., Mickey,  B., Nettleton,  J., and Howard,  J., 1994, “Flexural Rigidity of Microtubules and Actin Filaments Measured from Thermal Fluctuations in Shape,” J. Cell Biol., 120, pp. 923–934.
Love, A. E. H., 1952, A Treatise on the Mathematical Theory of Elasticity, Dover, New York, Fourth Edition.
Spector,  A. A., Brownell,  W. E., and Popel,  A. S., 1998, “Elastic Properties of the Composite Outer Hair Cell Wall,” Ann. Biomed. Eng., 26, pp. 157–165.
Brownell,  W. E., Spector,  A. A., Raphael,  R. M., and Popel,  A. S., 2001, “Micro- and Nanomechanics of the Cochlear Outer hair Cell,” Ann. Biomed. Eng., 3, pp. 169–194.
Dallos,  P., Hallworth,  R., and Evans,  B. N., 1993, “Theory of Electrically Driven Shape Changes of Cochlear Outer Hair Cells,” J. Neurophysiol., 70, pp. 299–323.
Dallos,  P., and He,  D. Z. Z., 2000, “Two Models of Outer Hair Cell Stiffness and Motility,” JARO, 1, pp. 283–291.


Grahic Jump Location
(a) General view of the outer hair cell cytoskeleton and (b) fragment of the cytoskeleton showing its multiple-domain nanostructure, longer filaments and shorter crosslinks inside domain, and strips of the intermediate material between domains
Grahic Jump Location
(a) Model of the domain with parallel filaments and inclined crosslinks and (b) model of the connection of neighboring domains: spring model in the case of side-by-side contact and double-truss model in the case of edge-to-edge contact
Grahic Jump Location
Deformation of a patch representing the effective properties of the cytoskeleton under the action of (a) load in the circumferential direction (parallel to sides 2 and 4), (b) load in the longitudinal direction (parallel to sides 1 and 3), and (c) shear load; finite element discretization is shown for the domains and intermediate material; thick and thin lines correspond, respectively, to the deformed and undeformed states.
Grahic Jump Location
Histograms of the anisotropic stiffness moduli of the outer hair cell cytoskeleton, including the mean values (μ) and standard deviations (σ); (a) modulus C11, (b) modulus C12, (c) modulus C13, (d) modulus C22, (e) modulus C23, and (f ) modulus C33



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