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TECHNICAL PAPERS

Effects of Static Stress on the Mechanical Properties of Cultured Collagen Fascicles From the Rabbit Patellar Tendon

[+] Author and Article Information
Ei Yamamoto

Laboratory on Mechanical Behavior of Materials, Department of Mechanical Engineering, School of Biology-Oriented Science and Technology, Kinki University, Wakayama 649-6493, Japane-mail: ei@fmec.waka.kindai.ac.jp

Wataru Iwanaga

Kubota Corporation, Osaka 590-0823, Japan

Hiroshi Miyazaki, Kozaburo Hayashi

Biomechanics Laboratory, Division of Mechanical Science, Department of Systems and Human Science, Graduate School of Engineering Science, Osaka University, Osaka 560-8531, Japan

J Biomech Eng 124(1), 85-93 (Aug 16, 2001) (9 pages) doi:10.1115/1.1427924 History: Received April 18, 2000; Revised August 16, 2001
Copyright © 2002 by ASME
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References

Figures

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Resection of collagen fascicles from a rabbit patellar tendon under sterile condition. Collagen fascicles having the diameter and length of approximately 300 μm and 15 mm, respectively, were carefully dissected from the patellar tendon with a surgical knife, while the tendon was immersed in Hanks’ balanced salt solution (HBSS).
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Apparatus for the culture of collagen fascicles under static load condition. Each fascicle was immersed in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10 percent fetal calf serum (FCS), 100 U/mL penicillin, and 100 μg/mL streptomycin. A stainless steel weight was suspended using a suture to apply static load to the fascicle. Four fascicles can be set up in the apparatus.
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Definition of crimp angle, crimp length, and wave length. Crimp length was defined as the distance between the top and bottom points of a fibril. Crimp angle was defined as the angle between the line connecting the top and bottom points and the line connecting neighboring two middle points. Wave length was calculated from the crimp angle and crimp length.
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Typical stress-strain curves of the fascicles cultured under different stresses for 1 (a) and 2 wk (b), and averaged curves of control and non-loaded fascicles. These stress-strain curves were almost linear between 2 and 5 percent strain, with toe regions under 2 percent strain.
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Applied stress versus tangent modulus of cultured collagen fascicles. There were statistically significant correlations between them in 1-wk, 2-wk, and pooled data; each relation was described by a quadratic curve. The quadratic function for the pooled data had the maximum modulus of 175 MPa at the applied stress of 1.3 MPa. Each point represents the average of the data from one to four fascicles.
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Applied stress versus tensile strength of cultured collagen fascicles. There were statistically significant correlations between them in 1-wk, 2-wk, and pooled data; each relation was described by a quadratic curve. The quadratic function for the pooled data had the maximum strength of 16.7 MPa at the applied stress of 1.2 MPa. Each point represents the average of the data from one to four fascicles.
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Applied stress versus strain at failure of cultured collagen fascicles. Strain at failure was negatively correlated with applied stress, and the relations were statistically significant. Each point represents the average of the data from one to four fascicles.
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Examples of the photomicrographs of crimp morphology in a control fascicle (A) and the fascicles cultured under no load (B), 0.44 MPa (C), 1.27 MPa (D), and 2.55 MPa (E) for 2 wk. Bars indicate 50 μm.

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