Chained Vesicles in Vascular Endothelial Cells

[+] Author and Article Information
T. Kosawada

Graduate School of Human Sensing and Functional Sensor Engineering, Yamagata University, Jonan 4-3-16, Yonezawa 992-8510, Japan

R. Skalak, G. W. Schmid-Schönbein

Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093-0412

J Biomech Eng 121(5), 472-479 (Oct 01, 1999) (8 pages) doi:10.1115/1.2835075 History: Received March 17, 1998; Revised April 20, 1999; Online January 23, 2008


There is extensive ultrastructural evidence in endothelium for the presence of chained vesicles or clusters of attached vesicles, and they are considered to be involved in specific transport mechanisms, such as the formation of trans-endothelial channels. However, few details are known about their mechanical characteristics. In this study, the formation mechanism and mechanical aspects of vascular endothelial chained vesicles are investigated theoretically, based on membrane bending strain energy analysis. The shape of the axisymmetric vesicles was computed on the assumption that the cytoplasmic side of the vesicle has a molecular layer or cytoskeleton attached to the lipid bilayer, which induces a spontaneous curvature in the resting state. The bending strain energy is the only elasticity involved, while the shear elasticity is assumed to be negligible. The surface area of the membrane is assumed to be constant due to constant lipid bilayer thickness. Mechanically stable shapes of chained vesicles are revealed, in addition to a cylindrical tube shape. Unfolding of vesicles into a more flattened shape is associated with increase in bending energy without a significant increase in membrane tension. These results provide insights into the formation mechanism and mechanics of the chained vesicle.

Copyright © 1999 by The American Society of Mechanical Engineers
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