Confocal Imaging of Flows in Artificial Venular Bifurcations

[+] Author and Article Information
D. L. Hitt, M. L. Lowe

Department of Physics, Loyola College, Baltimore, MD 21210

J Biomech Eng 121(2), 170-177 (Apr 01, 1999) (8 pages) doi:10.1115/1.2835099 History: Received September 24, 1997; Revised September 03, 1998; Online January 23, 2008


We describe a new experimental methodology for visualizing three-dimensional structures in microscopic tubes under flow conditions. Through the use of microfabrication techniques, artificial venular bifurcations are constructed from glass tubes with semicircular cross sections (radius = 50 μ). Aqueous fluorescent solutions are infused into the tubes at flow rates of about 1 μ1/min, a value comparable to blood flow in the microcirculation. The flow is imaged using a combination of confocal microscopy and three-dimensional image reconstruction software techniques. The quantitative accuracy of the experimental method is evaluated by measuring the “separation surface,” a formation resulting from converging flows at a bifurcation. Details of the fabrication process, fluidics, confocal microscopy, image reconstructions, optical effects, and computations are described. We show the first three-dimensional visualization of a microscopic flow structure using confocal microscopy, and within certain limitations, quantitative agreement between the measured and computed positions of the separation surface.

Copyright © 1999 by The American Society of Mechanical Engineers
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