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SPECIAL PAPERS ON MICROSYSTEMS TECHNOLOGY IN MEDICINE AND BIOLOGY

Microcontact Printing for Precise Control of Nerve Cell Growth in Culture

[+] Author and Article Information
B. C. Wheeler

Beckman Institute; Neuroscience Program; Department of Electrical and Computer Engineering

J. M. Corey

Beckman Institute; Neuroscience Program. University of Illinois at Urbana–Champaign, Urbana, IL 61801

G. J. Brewer

Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield, IL 62794

D. W. Branch

Beckman Institute; Biophysics Program; University of Illinois at Urbana–Champaign, Urbana, IL 61801

J Biomech Eng 121(1), 73-78 (Feb 01, 1999) (6 pages) doi:10.1115/1.2798045 History: Received July 22, 1998; Revised October 06, 1998; Online October 30, 2007

Abstract

Microcontact printing, facilitated by silane linker chemistry and high-relief stamps, creates precise patterns of proteins, which in turn control growth of hippocampal neurons in culture. This additive, multi-mask technique permits several different molecules to be patterned on the same substrate. The covalent linker technology permits relatively long-term (two-week) compliance of neurons to the stamped pattern against a polyethylene glycol background. When polylysine was stamped adjacent to a laminin/polylysine mixture, neural somata and dendrites preferred the polylysine while axons prefer the mixture or the border between the two.

Copyright © 1999 by The American Society of Mechanical Engineers
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