0
SPECIAL PAPERS ON MICROSYSTEMS TECHNOLOGY IN MEDICINE AND BIOLOGY

Surfaces Designed to Control the Projected Area and Shape of Individual Cells

[+] Author and Article Information
C. H. Thomas

Division of Biological Materials, Northwestern University Medical School, 311 E. Chicago Ave., Chicago, IL 60611-3008; Department of Biomedical Engineering, Robert R. McCormick School of Engineering & Applied Science, Northwestern University, Evanston, IL 60201

J.-B. Lhoest, D. G. Castner

NESAC/Bio, University of Washington, Departments of Chemical Engineering and Bioengineering, Box 351750, Seattle, WA 98195

C. D. McFarland

Department of Biomolecular Engineering, CSIRO, New South Wales, 2113 Australia

K. E. Healy

Division of Biological Materials, Northwestern University Medical School, 311 E. Chicago Ave., Chicago, IL 60611-3008; Department of Biomedical Engineering, Robert R. McCormick Schooi of Engineering & Applied Science, Northwestern University, Evanston, IL 60201

J Biomech Eng 121(1), 40-48 (Feb 01, 1999) (9 pages) doi:10.1115/1.2798041 History: Received July 08, 1998; Revised October 01, 1998; Online October 30, 2007

Abstract

Materials with spatially resolved surface chemistry were designed to isolate individual mammalian cells to determine the influence of projected area on specific cell functions (e.g., proliferation, cytoskeletal organization). Surfaces were fabricated using a photolithographic process resulting in islands of cell binding N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane (EDS) separated by a nonadhesive interpenetrating polymer network [poly (acrylamide-co-ethylene glycol); P(AAm -co-EG)]. The surfaces contained over 3800 adhesive islands/cm 2 , allowing for isolation of single cells with projected areas ranging from 100 μm2 to 10,000 μm2 . These surfaces provide a useful tool for researching how cell morphology and mechanical forces affect cell function.

Copyright © 1999 by The American Society of Mechanical Engineers
Your Session has timed out. Please sign back in to continue.

References

Figures

Tables

Errata

Discussions

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging and repositioning the boxes below.

Related Journal Articles
Related eBook Content
Topic Collections

Sorry! You do not have access to this content. For assistance or to subscribe, please contact us:

  • TELEPHONE: 1-800-843-2763 (Toll-free in the USA)
  • EMAIL: asmedigitalcollection@asme.org
Sign In