A Tissue-Like Culture System Using Microstructures: Influence of Extracellular Matrix Material on Cell Adhesion and Aggregation

[+] Author and Article Information
G. Knedlitschek, F. Schneider, E. Gottwald, E. Eschbach, K. F. Weibezahn

Institute of Toxicology, Karlsruhe Research Center, P.O. Box 3640, D-76021 Karlsruhe, Federal Republic of Germany

Th. Schaller

Central Experimentation Engineering Department, Karlsruhe Research Center, P.O. Box 3640, D-76021 Karlsruhe, Federal Republic of Germany

J Biomech Eng 121(1), 35-39 (Feb 01, 1999) (5 pages) doi:10.1115/1.2798040 History: Received May 28, 1998; Revised October 01, 1998; Online October 30, 2007


Special microenvironmental conditions are required to induce and/or maintain specific qualities of differentiated cells. An important parameter is the three-dimensional tissue architecture that cannot be reproduced in conventional monolayer systems. Advanced tissue culture systems will meet many of these demands, but may reach their limits, especially when gradients of specific substances over distinct tissue layers must be established for long-term culture. These limitations may be overcome by incorporating microstructures into tissue-like culture systems. The microstructured cell support presented consists of a flat array of 625 cubic microcontainers with porous bottoms, in which cells can be supplied with specific media from both sides of the tissue layer. Permanent cell lines and primary rat hepatocytes have been used to test the culture system. In order to define reproducible conditions for tissue formation and for cell adherence to the structure, several ECM (extracellular matrix) components were tested for coating of microstructured substrata. The described tissue culture system offers great flexibility in adapting the cell support to specific needs.

Copyright © 1999 by The American Society of Mechanical Engineers
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