A Method to Study Intracellular Ice Nucleation

[+] Author and Article Information
K. Tatsutani, B. Rubinsky

Biomedical Engineering Laboratory, 6178 Etcheverry Hall, Department of Mechanical Engineering, University of California at Berkeley, Berkeley, CA 94720

J Biomech Eng 120(1), 27-31 (Feb 01, 1998) (5 pages) doi:10.1115/1.2834302 History: Received September 09, 1996; Revised January 23, 1997; Online January 07, 2008


The thermodynamics of intracellular ice nucleation are important in low-temperature biology for understanding and controlling the process of cell destruction by freezing. We have developed a new apparatus and technique for studying the physics of intracellular ice nucleation. Employing the principle of directional solidification in conjunction with light microscopy, we can generate information on the temperature at which ice nucleates intracellularly as a function of the thermal history the cells experience. The methodology is introduced, and results with primary prostatic cancer cells are described.

Copyright © 1998 by The American Society of Mechanical Engineers
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