The Measurement of Temperature With Electron Paramagnetic Resonance Spectroscopy

[+] Author and Article Information
J. J. Eckburg, J. C. Chato, K. J. Liu, M. W. Grinstaff, H. M. Swartz, K. S. Suslick, F. P. Auteri

Departments of Mechanical and Industrial Engineering, Chemistry, and Internal Medicine, University of Illinois at Urbana-Champaign, Urbana, IL 61801

J Biomech Eng 118(2), 193-200 (May 01, 1996) (8 pages) doi:10.1115/1.2795959 History: Received December 31, 1993; Revised November 05, 1995; Online October 30, 2007


An electron paramagnetic resonance (EPR) technique, potentially suitable for in vivo temperature measurements, has been developed based on the temperature response of nitroxide stable free radicals. The response has been substantially enhanced by encapsulating the nitroxide in a medium of a fatty acid mixture inside a proteinaceous microsphere. The mixture underwent a phase transition in the temperature range required by the application. The phase change dramatically altered the shape of the EPR spectrum, providing a highly temperature sensitive signal. Using the nitroxide dissolved in a cholesterol and a long-chain fatty acid ester, we developed a mixture which provides a peakheight ratio change from 3.32 to 2.11, with a standard deviation of 0.04, for a temperature change typical in biological and medical applications, from 38 to 48°C. This translated to an average temperature resolution of 0.2°C for our experimental system. The average diameter of the nitroxide mixture-filled microspheres was ≈2 μm. Therefore, they are compatible with in vivo studies where the microspheres could be injected into the microvasculature having a minimum vessel diameter of the order of 8 μm. This temperature measuring method has various potential clinical applications, especially in monitoring and optimizing the treatment of cancer with hyperthermia. However, several problems regarding temperature and spatial resolution need to be resolved before this technique can be successfully used to monitor temperatures in vivo.

Copyright © 1996 by The American Society of Mechanical Engineers
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