Use of Micropathways to Improve Oxygen Transport in a Hepatic System

[+] Author and Article Information
Randall E. McClelland, Robin N. Coger

Department of Mechanical Engineering & Engineering Science, University of North Carolina—Charlotte, 9201 University City Blvd., Charlotte, NC 28223-0001

J Biomech Eng 122(3), 268-273 (Feb 06, 2000) (6 pages) doi:10.1115/1.429657 History: Received October 21, 1999; Revised February 06, 2000
Copyright © 2000 by ASME
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Schematic of oxygen transport in the control system (a) and the “modified” double-walled collagen configuration (b, c). Collagen layers (gray sections) sandwich the hepatocytes, and the cell medium is placed on top (wavy lines). As indicated, the main direction of O2 flow (arrows) is through the medium in the control system (a), and radially outward from the central tube in the “modified” configuration (b, c). A top view of the “modified” configuration is shown in Fig. 1(c).
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Schematic of the cellular oxygen assay used to sustain hepatocytes in the double wall configuration. A nonpermeable container encapsulates the modified double-wall cultures, such that pressurized O2 reaches the cells by diffusing through the central tube (Fig. 1(b)). To ensure suitable CO2 levels, a perfusion system circulates cell medium percolated with CO2, across the surface of the culture.
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Reverse polarity micrographs of O2 transport in normal (A, B) and enhanced (C, D) collagen gels using fluorescent oxygen quenching dye particles
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Schematic of the radial distance from the O2 source within which viable hepatocytes can be sustained for the “modified” (gray) and “modified enhanced” (gray & white) configurations
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Albumin secretion rate of the collagen system control as compared to literature results
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A comparison of albumin secretions between the functional control, the “modified,” “enhanced modified,” and “enhanced modified double-wall” configurations
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Comparison of urea concentration levels of the functional control, the “modified,” “enhanced modified,” and “enhanced modified double-wall” configurations
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Normalized albumin secretion rates of the functional control, the “modified,” “enhanced modified,” and “enhanced modified double-wall” configurations




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