Mechanisms of Stiffening and Strengthening in Media-Equivalents Fabricated Using Glycation

[+] Author and Article Information
T. S. Girton

Departments of Chemical Engineering & Materials Science and Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455-0132

T. R. Oegema

Orthopaedic Surgery, University of Minnesota, Minneapolis, MN 55455-0132

E. D. Grassl, B. C. Isenberg, R. T. Tranquillo

Departments of Chemical Engineering & Materials Science and Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455-0132

J Biomech Eng 122(3), 216-223 (Feb 06, 2000) (8 pages) doi:10.1115/1.429652 History: Received November 18, 1999; Revised February 06, 2000
Copyright © 2000 by ASME
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Grahic Jump Location
Evolution of dry weight and collagen content of MEs: MEs incubated in standard medium for up to 10 weeks had a transient increase in dry weight but no increase in collagen content
Grahic Jump Location
Stress–strain comparison of glycated ME and arterial tissue: Circumferential stress–strain curves for MEs incubated for 6 weeks in standard medium supplemented with 30 mM ribose are more comparable to that measured for a freshly harvested abdominal aorta from an adult Fisher rat
Grahic Jump Location
Stress–strain curves for MEs fabricated with high versus low passage SMCs: Circumferential stress–strain curves for MEs fabricated with adult SMCs at passage 5 (“low”) or passage 30 (“high”) and incubated for 13 weeks in standard medium supplemented with 50 μg/ml ascorbic acid reveal MEs fabricated with the low passage adult SMCs were considerably stiffer and stronger




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