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RESEARCH PAPERS: Papers on Cell Biomechanics

Determination of the Red Blood Cell Apparent Membrane Elastic Modulus From Viscometric Measurements

[+] Author and Article Information
A. Drochon, D. Barthes-Biesel

Université de Compiègne, U.A. CNRS 858, BP 649 60206 Compiègne Cedex France

C. Lacombe, J. C. Lelievre

Unité de Biorhéologie, CHU Pitié-Salpétrière, Université Paris 6, CNRS URA 343 91, Bd de l’Hopital 75013 Paris, France

J Biomech Eng 112(3), 241-249 (Aug 01, 1990) (9 pages) doi:10.1115/1.2891179 History: Received January 02, 1990; Revised May 15, 1990; Online March 17, 2008

Abstract

Rhelogical measurements on a dilute suspension of red blood cells (RBCs) are interpreted by means of a microheological model that relates the shear evolution of the apparent viscosity to the intrinsic properties of the suspended particles. It is then possible to quantify the average deformability of a RBC population in terms of a mean value of the membrane shear elastic modulus, Es . Dilute suspensions of erthrocytes exhibit a shear-thinning behavior with a constant high shear viscosity. This behavior is identical to the one predicted for a suspension of spherical capsules where the same phenomena of deformation and orientation prevail. A comparison between theoretical and experimental curves yields a mean value of Es , assuming all other cell properties—internal viscosity, geometry—to be otherwise equal. In Dextran, the values of Es for normal RBCs are found to be of order 3.10−6 N/m. For erythrocytes hardened by heat exposure for 15 minutes at 48°C, the increase in Es reaches 45 percent. This procedure of shear elastic modulus determination is easy to perform and seems to give a good discrimination between normal and altered erythrocytes.

Copyright © 1990 by The American Society of Mechanical Engineers
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