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RESEARCH PAPERS

Analysis of the Water Permeability of Human Granulocytes at Subzero Temperatures in the Presence of Extracellular Ice

[+] Author and Article Information
G. J. Schwartz, K. R. Diller

Department of Mechanical Engineering, Biomedical Engineering Center, The University of Texas at Austin, Austin, Tex. 78712

J Biomech Eng 105(4), 360-366 (Nov 01, 1983) (7 pages) doi:10.1115/1.3138433 History: Received February 04, 1983; Revised July 11, 1983; Online June 15, 2009

Abstract

The plasma membrane water permeability of human granulocytes in the presence of extracellular ice was determined experimentally on a cryomicroscope. Transient volumes of individual cells were measured at constant subzero temperatures subsequent to ice nucleation. Permeability values were deduced by adjustment of multiple parameters in a model to obtain an optimal fit to the data. The permeability was determined to be a function of both temperature and intracellular solute osmolality, with a reference value at 0°C of 0.407 μm/atm·min and temperature and solute coefficients of 218kJ/mol and 1.09 Osm/kg.

Copyright © 1983 by ASME
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